Fermentation monitoring and control by on-line flow injection and liquid chromatography

Abstract The on-line use of flow-injection analysis to determine monosaccharides and disaccharides (in the presence and absence of glucose), lactate, amino acids and glutamine during the production of alkaline protease and penicillin and their use in process control are reported. The results of the use of on-line and off-line liquid chromatography in the determination of cephalosporins, penicillins, carbohydrates, carboxylic and amino acids and inorganic anions during the production of extracellular enzymes by bacteria, intracellular fusion proteins by means of recombinant bacteria and antibiotics by fungi and their use in process control are also reported.