Deconstructing Molecular Phylogenetic Relationship Among Cultivated and Wild Brassica Species

Brassica represents an agriculturally important and diverse group of oilseed crops with a long evolutionary history. Molecular markers played an important role in understanding origin and evolution of Brassica species. In the present research, both Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) developed from Brassica juncea L. Czern. were used to find out the phylogenetic relationship between different cultivated and wild Brassica species. A total of 88 SSR and 58 SNP markers were found to be functional across 38 genotypes belonging to ten different taxon groups. The polymorphic markers were able to group the genotypes into three different clusters and showed relatedness among different genomes based on genetic distances. The transferability of these markers serve the purpose of their quick use in cultivar identification, diversity and phylogenetic analysis in those orphan crops species where no or less genomic information is available.