Microparticle Signature for Acute Coronary Syndrome (ACS) by Polarization Flow Cytometry

Microparticles have been of keen interest in biomedical science for last few decades. The correlation of microparticles with different disease profiles, e.g. platelet mediated blood cell disorder, is well known. In this study we aim to use a microparticle based disease marking system based on pro-activator like properties of nanoparticles. Challenging platelet cells with nanoparticles changes their microparticle release profile. The extent of this release can in turn serve as a disease marker for Acute Coronary Syndrome (ACS) patients. Age and sex matched ACS and non-ACS patients have been chosen as subjects. Platelet rich plasma from both case and control groups have been isolated and allowed to interact with gold nanoparticle. For quantification of data a parallel computational method is employed using Matlab Engine. The analysis helped image based representation of the ACS specific microparticle release and their modulation by gold nanoparticle. The polarization flowcytometry is used as label free microparticle marker. The analyses reveal a significant difference in the microparticle population between ACS and non-ACS individuals. The differential of the release is further amplified in presence of gold nanoparticles. It has been observed that there is some significant difference (p value= 0.036) in the production of microparticles between ACS and non-ACS individuals after GNP incubation and this difference between cases and controls can be used further as a signature for ACS. To our knowledge the imaging aided flowcytometric method has for the first time enabled micropatricle classification based on polarization scattering.