Pregnane glycosides from the antidepressant active fraction of cultivated Cynanchum otophyllum

Abstract Based on the bioactive screening results, four new pregnane glycosides, namely cynanotophyllosides A–D ( 1 – 4 ) were isolated from the anti-depressant active fraction of cultivated Cynanchum otophyllum , along with thirteen known compounds ( 5–17 ). The new compounds were characterized as qingyangshengenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-oleandropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside ( 1 ), qingyangshengenin-3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranosyl-(1 → 4)-β-D-oleandropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-α-L-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside ( 2 ), caudatin-3-O-β-D-glucopyranosyl-(1 → 4)-β-D-thevetopyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-digitoxopyranoside ( 3 ) caudatin-3-O-β-D-glucopyranosyl -(1 → 4)-β-D-thevetopyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside ( 4 ), by detailed spectroscopic analysis and acidic hydrolysis.