Regulation on cell cycle and proliferation invasion of SEPT7 on glioma cell line U251

Objective To investigate the effect of SEPT7 on cell cycle and proliferation, invasion, apoptosis of gliomas cells U251. Methods The cell proliferation was determined by MTT assay and flowcytometry. cell regulators were detected by western blot, cell apoptosis was detecteded with Annexin V staining and cell invasion was evaluated by motility in three-dimensional culture. Results It was found that after transfection with SEPT7, the proliferation activity of U251 cell was inhibited, cell cycle was arrested in G0/G1 phase and the S phase fraction (SPF) was lowered. SEPT7 also increased apoptotie cells and attenuated the invasive ability of U251 cell. Conclusion SEPT7 inhibited tumor growth and invasion, and induced cell apoptosis in vivo. These results reveal that SEPT7 exerted the suppressive effect on the glioma cell growth and invasion, induced apoptosis, and suggested that SEPT7 as a suppressor gene of glioma. Key words: Glioma;  SEPT7;  Cell cycle;  Apoptosis