Solubilization of udp-glucose collagen glucosyltransferase activities from chick embryo liver: Golgi apparatus, smooth and rough endoplasmic reticulum

Abstract 1. 1. The choice of a suitable detergent for solubilization of UDP-glucose collagen glucosyltransferase (GGT) activities from chick embryo liver has been investigated. Several detergents were used (zwitterionic detergent as Chaps, and non-ionic detergents as Triton X-100, Nonidet P 40, Brij 35). 2. 2. All the detergents with GGT activities were tested in Golgi apparatus, smooth and rough endoplamic reticulum (SER, RER). 3. 3. 80–100% GGT Golgi apparatus activity was easily solubilized at low concentrations in surfactant (0,5 mg/ml). 25–78% of SER and RER GGT activities were extracted at this concentration. 4. 4. A higher level of detergent (5 mg/ml) was necessary to release all GGT activities of SER and RER. Protein extraction was identical to GGT activities.