Analysis of microdeletions of azoospermia factor on the Y chromosome in male infertile patients

Objective: To investigate the relationship between microdeletion of azoospermia factor(AZF) and male infertility.Methods: Multiplex PCR was used to detect Y chromosome microdeletion in AZFa,AZFb and AZFc of 118 cases of idiopathic azoospermia,84 cases of severe idiopathic oligozoospermia,and 66 cases of healthy male controls.Results: No microdeletion was found in 66 controls.Y chromosome microdeletion was found in 25 of 202 azoospermia patients,the total p revalence rate of microdeletion was 12.4%.There were 17 cases(12 for azoospermia,5 for severe oligozoospermia) in AZFc(8.4%);3 cases(1 for azoospermia,2 for severe oligozoo spermia) in AZFb+c(1.5%);1 case(1 for azoospermia) in AZFa+b+c(0.5%).According to statistics,the difference between two groups was significant(P0.001).Conclusion: Y chromosome microdeltions is an important reason for azoospermia,and the screening of Y chromosome microdeletions for azoospermia patients before ICSI treatment is essential.