Analysis of estrogenicity, toxicity and neurotoxicity ofMicrocystis aerigunosa, Cylidrospermopsis raciborskii andScenedesmus quadricauda exudates with zebrafish (Danio rerio)embryos.
2012
Cyanobacteria are known to synthesize mixture of diverse
biologically active compounds which are partially excreted into
natural water bodies. The structural identity as well as
potential biological activity and toxicological properties of
many of these compounds are still unknown. In this study the
zebrafish embryo model was used to investigate the potential
estrogenicity, neurotoxicity and general toxicity of exudates
from laboratory cultures of two cyanobacteria species
Microcystis aerigunosa and Cylidrospermopsis raciborskii and
one green algae Scenedesmus quadricauda. Exudate samples were
collected after 21-day exponential growth separating the liquid
phase from cells; after that the liquid phase was extracted by
solid phase extraction. A transgenic zebrafish strain
(cyp19a1b-GFP, Brion et al., PloS ONE 2012, e36069) was used to
assess the estrogenicity. This strain expresses green
fluorescent protein under the control of aromatase B (cyp19a1b)
promoter, which is induced by estrogens. The effects of
exposure to exudates on locomotor response were used as an
indicator of potential neurotoxic effects. A significant
increase in estrogenicity compared to control was observed in
embryos exposed to 10 times concentrated exudate of Microcystis
aerigunosa and 10 and 17 times concentrated exudate of
Scenedesmus quadricauda. Phenotypic toxicity was not observed
in any concentration of Scenedesmus quadricauda (1 - 17 times
concentrated) exudate. However, in case of exudates of
Microcystis aerigunosa deformation of tip of the tail appeared
from 3.3 times concentrated samples; all embryos in 10 times
concentrated exudates had deformities of the tail and
frequently also heart edemas and lethality occurred in 17 times
concentrated samples. The results of ongoing work on
Cylidrospermopsis raciborskii, neurotoxicity and other
endpoints will be also presented.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI