Development of PCR Detection Method for Sacbrood Virus in Honeybee (Apis mellifera L.)

A PCR method was developed to detect and verify Sacbrood Virus (SBV), which affects to honeybee larvae. The PCR assay was performed with SBV specific primer set and amplified 824bp fragment from SBV genome. The SBV amplicon containing a BamHI restriction site was digested by this enzyme for a brief verification of SBV infection. SBV3-842F/R primer set showed high specificity and sensitivity in detection of SBV. SBV amplicon was cloned and sequenced to make sure result of PCR and digestion. It showed 94% and 99% homology in nucleotide and amino acid sequence with SBV complete genome (AF092924), respectively. In this paper, we report a specific SBV primer pairs for detection and verification of SBV.