A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat

Abstract Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO 4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, P pl , F res , C dyn , and R i ) and cardiovascular function (BP and F heart ) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8–10 min) bron-choconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21–150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo.