Effects of pentoxifylline on Wnt/β-catenin signaling in mice chronically exposed to cigarette smoke.

Background Previous discovery that long-term administration of pentoxifylline (PTX) to mice chronically exposed to smoke led to the development of pulmonary fibrosis rather than emphysema initiated our curiosity on whether the Wnt/β-catenin pathway, a set of signaling proteins essential to organ development and lung morphogenesis in particular were activated in the pathogenesis of pulmonary fibrosis. Methods Male BALB/c mice were randomized into four study groups: Group Sm, smoke exposure and taken regular forage; Group PTX, no smoke but taken PTX-rich forage; Group Sm+PTX, smoke exposure and taken PTX-rich forage; Group control: shamed smoke exposure and taken regular forage. Animals were sacrificed at day 120. Morphometry of the lung sections and the expressions of TGF-β 1 , hydroxyproline, β-catenin, cyclin D1, T cell factor 1 (Tcf-1) and lymphoid enhancer factor 1 (Lef-1) mRNA, etc, in the lung homogenate or in situ were qualitatively or quantitatively analyzed. Results As expected, smoke exposure along with PTX administration for 120 days, lungs of the mice progressed to be a fibrosis-like phenotype, with elevated fibrosis score (3.9±1.1 vs. 1.7±0.6 in Group Sm, P <0.05). TGF-β 1 (pg/g) (1452.4±465.7 vs. 818.9±202.8 in Group Sm, P <0.05) and hydroxyproline (mg/g) (5.6±0.6, vs. 2.4±0.1 in Group Sm, P <0.05) were also consistently increased. The upregulation of β-catenin measured either by counting the cell with positive staining in microscopic field (17.4±7.9 vs. 9.9±2.9 in Group Sm, P <0.05) or by estimation of the proportion of blue-stained area by Masson's trichrome (11.8±5.6 vs. 4.7±2.4 in Group Sm) in Group SM+PTX was much more noticeable as than those in Group Sm. The expression of β-catenin measured by positive cell counts was correlated to TGF-β 1 concentration in lung tissue (r=0.758, P <0.001). PTX per se caused neither fibrosis nor emphysema though expression of β-catenin and downstream gene cyclin D 1 may also be altered by this medication. Conclusions PTX mediated transformation of pulmonary emphysema into pulmonary fibrosis under chronic cigarette smoke exposure is associated with upregulation of β-catenin and elevation of TGF-β 1 , implying that activation of Wnt/β-catenin signaling may be involved in the pathogenesis of pulmonary fibrosis.