Optimization of Culture Medium for a New Tetrodotoxin-producing Marine Bacterium B3B by Uniform Design
2007
A new tetrodotoxin-producing marine bacterium, B3B was isolated from the ovary of puffer fish Fugu rubripes collected from the Bohai Sea, and the tetrodotoxin (TTX)was identified by high-performance liquid chromatography (HPLC), mass spectrometry (MS) and mouse toxicity assay. UROS software was used to choose the optimum medium constituents. The TTX yield from the optimized medium was increased by 128.7%, compared to the original medium. Fig 2, Tab 2, Ref 21
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