Synergic Mutagenicity of NNK and B[a]P in Matrix of Cigarette Smoke

Taking B[a]P(benzo[a]pyrene)and NNK [4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone] as the target compounds,setting the concentration of cigarette smoke condensate(CSC)at 0(CK),0.005,0.010,0.015,0.020 mg/mL,that of NNK at 0(CK),0.005,0.010,0.020,0.040 mg/mL and that of B[a]P at 0(CK),0.00025,0.00050,0.00075,0.00100 mg/mL,the synergic mutagenicity of the three components was evaluated.The results showed that: 1)CSC,NNK and B[a]P all induced mutation of Salmonella typhimurium strain TA98 and TA100 with obvious dose response.2)There existed obvious synergism between B[a]P and CSC;high doses of B[a]P and NNK and low doses of CSC and NNK produced a weak additive effect.3)Under different conditions,the combined effects induced by the three components on TA98 showed weak additive effect.By and large,the unified mutagenicity of NNK and B[a]P in the matrix of cigarette smoke showed the complexity of dose-response,time-response and sequence of action.