택사의 항비만 효과 및 항산화 효과

Objectives : The aim of this study is to investigate the inhibitory effect of pancreatic lipase and its anti-oxidant effect of Alisma orienta water extract (AOE). Methods : Alisma orienta (30 g) was extracted 10 times with distilled water (300 ㎖) for 2h. The inhibitory activity on pancreatic lipase enzyme of AOE was evaluated in vitro and its anti-oxidant effects were studied based on 2.2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and 2,2ʹ-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. In addition, total phenolic content was measured as gallic acid equivalents (GAE) by the reference and the flavonoid content was measured as naringin equivalent (NE) by the reference. Results : In our current study, the obtained result showed that AOE inhibited pancreatic lipase activity with IC50 of below 1 ㎍/㎖. The IC50 of DPPH radical scavenging activity of AOE was 489.19 ± 1.02 ㎍/㎖ and the IC50 value of ascorbic acid as a positive control was 1.17 ± 0.04 ㎍/ ㎖. Moreover, the calculated IC50 value of AOE against the ABTS radical was 120.04 ± 1.67 ㎍/㎖ and the IC50 value of ascorbic acid as a positive control was 2.27 ± 0.19 ㎍/㎖. Total phenolic content was found to 6.29 ± 0.05 ㎎ GAE/g of AOE. The flavonoid content was 1.56 ± 0.02 ㎎ naringin equivalent (NE)/g of AOE. Conclusions : Taken together, AOE may exert anti-obesity effect by directly inhibiting pancreatic lipase, which would limit the lipid absorption from the small intestine. Accordingly, AOE administration may attenuate the physiological lipid changes caused by obesity.