Evaluation of the impact of suspended particles on the UV absorbance at 254 nm (UV254) measurements using a submersible UV-Vis spectrophotometer.

There is an increasing need to use online instrumentation for continuous monitoring of water quality. However, industrial applications using online instruments, such as submersible UV-Vis spectrophotometers, may require the use of alternative techniques to remove particle effect rather than performing a physical filtration step. Some submersible UV-Vis spectrophotometers have built-in generic particle compensation algorithms to remove the filtration step. This work studied the influence of suspended particles on the measurements of a submersible UV-Vis spectrophotometer as well as the performance of the built-in particle compensation technique under laboratory-controlled conditions. Simulated water samples were used in the combinations of standard particles from laboratory chemical and natural particles extracted from water systems with ultrapure water and treated water from a drinking water treatment plant. Particle contributions to the UV absorbance at 254 nm (UV254) measurements of water samples varied differently when particle types or concentrations changed. The compensated UV254, measured by the submersible instrument using the built-in generic particle compensation algorithms, was compared with laboratory UV254, analysed by the bench-top instrument with the physical filtration method. The results indicated that the built-in generic compensation algorithms of the submersible UV-Vis spectrophotometer may generate undercompensated UV254 or overcompensated UV254 for various surface waters. These findings provide in-depth knowledge about the impact of suspended particles on the measurements of submersible UV-Vis spectrophotometers; source water dependence; and why site-specific calibration is often needed to get accurate measurements.