Molecular Characterization of a Rhodotorula-lytic Enzyme from Paecilomyces lilacinus Having β-1,3-Mannanase Activity

We cloned the gene and corresponding cDNA for an extracellular Rhodotorula-lytic enzyme which has β-1,3-mannase activity, tentatively named MAN5C, from Paecilomyces lilacinus. MAN5C showed a high homology score with the members of glycoside hydrolase family 5 in a domain search with the Pfam database, indicating that MAN5C is a novel and unique member of glycoside hydrolase family 5.