X-100 method for measuring the residual amount of Triton HBsAg recombinant expression stock S. cerevisiae

The present invention discloses a recombinant HBsAg expression in Saccharomyces cerevisiae dope Triton? The method of measuring residual amounts of X-100, with a reference solution comprising a formulation step, the step of formulating the test solution and Triton? X-100 measuring step, the step of the test solution formulation comprising: a protein content of each test article in the detection, screening the protein content is greater than a threshold value of the test product and the test protein content of less than the threshold value products; respectively 1 part water by solid phase extraction column, for each test product for the first wash heteroaryl; protein content greater than the threshold value the test products, then 1 part water by solid phase extraction column, for for each test product wash again heteroaryl; for each test product for the third washing solution heteroaryl with 1 part by volume of methanol at a concentration of 2% to 70% by solid phase extraction column. The present invention can effectively remove protein and other interfering substances macromolecules stock solution of Triton? X-100 affect the determination of the amount of residual, high recovery rate can be measured accurately dope Triton? X-100 residues, which may reach the detection limit 1μg / ml level.