[Full sequence analysis for a null allele of MICA gene (MICA*063N)].

[Objective]To analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA).[Methods] A sequence-based typing method was used to determine the nucleotide sequence of the MICA gene.Potential alleles were identified with a computer program.[Results] The identified allele has possessed a sequence similar to that of MICA * 027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2.As a stop codon,this may result in a truncated protein.[Conclusion] A null allele of MICA gene has been identified.The sequence has been submitted to the Genbank nucleotide sequence database (submission No.HWS10011131),which was officially named as MICA * 063N by the WHO Nomenclature Committee in October 2010. Key words: MICA gene; Major histocompatibility complex class Ⅰ chain-related A * 063N; New allele; Sequence-based typing