A COMPARATIVE STUDY OF NEUROPROTECTIVE EFFECT OF TELMISARTAN AND DONEPEZIL AGAINST LIPOPOLYSACCHARIDE INDUCED NEUROINFLAMMATION IN MICE

Objective: The aim of the present study was a comparative study of neuroprotective effect of telmisartan and donepezil against lipopolysaccharide (LPS)-induced neuroinflammation in mice. Methods: In this study, we investigated the comparative effect of telmisartan (5 mg/kg, p.o.) and donepezil (5 mg/kg, p.o.) in systemic inflammation induced by LPS, ibuprofen (40 mg/kg, p.o.) was used as standard. Mice were treated with a single i.c. injection of LPS (5 µg/5 µl/kg), after 7 days the animal behavior was evaluated by testing specific cognitive functions, on Morris water maze and Pole climbing test. Biochemical estimation for glutathione (GSH), malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) was done by enzyme-linked immunosorbent assay plate reader. Results: The neuroprotective effect of telmisartan (5 mg/kg) and donepezil (5 mg/kg) in LPS induced neuroinflammation in mice was compared. Oral administration of telmisartan (5 mg/kg) for 7 days shows a better result in Morris water maze and pole climbing test, in comparison of donepezil. It also increases the level of GSH and decreases the level of MDA and TNF-α in mice brain. Conclusion: The present study demonstrates that telmisartan and donepezil reduces LPS-induced microglial activation, beta-amyloid generation, central nervous system cytokine production, and behavioral symptoms of sickness. In comparative study of telmisartan and donepezil, telmisartan shows significant decrease in escape latency time and transfer latency time in comparison of donepezil. Therefore, telmisartan is more effective as the comparative of donepezil. Keywords: Neuroprotective, Telmisartan, Donepezil, Lipopolysaccharide-induced neuroinflammation.