Differential Response to the High Doses of Dimethyl Sulfoxide of the Several Human Cancer Cell Lines Cultured in 2D Monolayer, Decellularized Matrix, and 3D Spheroid cell Culture Systems

When using cell-based models for drug research screening it is of critical importance to validate their applicability and accuracy. Here we report that the patterns of cellular response to high concentrations of DMSO measured with vital dye are cell line-specific and varied in time and type of response for different cell lines and for the same cell line cultures in 2D Monolayer, Decellularized Matrix, or 3D Spheroid cell culture systems. This should be considered at the stage of the experimental design, and for each experiment the more suitable cell culture system should be chosen, based on the characteristics of cellular response to DMSO in this particular system, especially if developing cell-based automated systems for drug screening.