Studies on trifluoromethylphosphonamidite analogues as building blocks in oligonucleotide synthesis

Abstract The phosphorylating reagents CF 3 P(NMe 2 )Cl and CF 3 P(NEt 2 )Cl are used to phosphorylate the 3′-hydroxyl moiety of several protected 2′-deoxynucleosides yielding the corresponding nucleoside trifluoromethyl phosphonamidites. Their scope and limitations towards amidite activation are investigated but, however, their behavior is completely different to commonly used nucleoside phosphorus amidites.