Comparative Genotoxicity of Two Fungicide Formulations in Meristematic Root Cells of Narcissus Pseudonarcissus Cv. 'Samanta'

The genotoxicity of the contact fungicide Dithane M 45 and systemic fungicide Tilt 250 EC were compared in meristematic root cells of Narcissus pseudonarcissus cv. ‘Samanta’. Irrespective of the concentration or sampling time, an increased mitodepressive and genotoxic potential was observed for Tilt 250 EC. The genotoxicity of both fungicide formulations was measured by analysis of the frequency of chromosomal aberrations in meristematic root cells. The increased chromosomal aberration frequency along with mitotic inhibition after treatment of meristematic root cells with Tilt 250 EC was supported by the mode of action of this systemic fungicide. Various types of chromosomal and mitotic abnormalities such as Cmetaphase, binucleated cells, laggard, disturbed anaphase showed in treated meristem cells reveal the clastogenic potential of both pesticides tested.