ДЕЙСТВИЕ АНТИБАКТЕРИАЛЬНЫХ ПРЕПАРАТОВ НА БИОПЛЕНКИ ХОЛЕРНЫХ ВИБРИОНОВ ЭЛЬ ТОР

Aim. Study the effect of antibacterial preparations on biofilms of Vibrio cholerae El Tor. Materials and methods. Sensitivity of V. cholerae El Tor (6 strains) to various concentrations of antibacterial preparations (doxycycline, tetracycline, levomycetin, rifampicin, gentamycin, ceftazidime) was determined (MD 4.2.2495-09). Transmission electron microscopy was used for visualization of the effect of preparations on biofilms. Results. The values of minimal inhibiting concentrations of antibacterial preparations against biofilms have increased by 5 - 100 times compared with plankton cultures. Certain smoothing of strands between the bacterial cell and substrate, alteration of vibrios’ form, reduction of electron density of the matrix with an increase of its transparency were observed during electron-microscopy of the effect of antibacterial preparations on the biofilm. Conclusion. Study of the effect of antibacterial preparations on biofilms could increase effectiveness of rational antibiotics therapy of infections by selection of preparations that disrupt functioning of microbial communities.