COMPARATIVE ANALYSIS OF THE GROWTH OF HELICOBACTER PYLORI IN DIFFERENT BLOOD AGAR MEDIUM

Objective: Helicobacter pylori is the gram negative pathogenic bacteria mainly associated with gastrointestinal disorders. The growth of the H. pylori has been achieved only by inoculating the clinical specimen and storage in blood agar only. The objective of this research was to compare the growth of H. pylori in different blood agar medium to elucidate whether the medium provided considerable growth of H. pylori in vitro. Methods: H. pylori was inoculated in human, goat, sheep, fish and chicken blood agar plates and incubated in anaerobic under microaerophilic (5% O 2 , 10% CO 2 and 85% N 2 ) conditions at 37°C for 4 days. After successful incubation, significant differences in growth was observed and further confirmed based on colony morphology, Gram staining and biochemical tests. Results: In human blood agar plates, it was observed that creditable colony size and growth of H. pylori. In goat blood also showed more or less equal results same like human blood; whereas minimal growth was observed in chicken, and no growth was observed in sheep and fish blood agar plates. Even though the                 H. pylori colonies were observed on chicken blood agar plates, the urease test of the colonies are very slow reactive than human and goat blood agar. But other parameters and characterization of goat blood agar are more or less equal like human blood. Conclusion: No previous report observed in the determination of H. pylori growth in different blood culture medium. Thus, this study is suggesting the usage of goat blood as the alternative for the human blood agar for                     H. pylori isolation and subculturing