The Measurement of the Absorption Spectra of Chlorine e 6 Photosensitizer and Hemoglobin in Whole Blood by Multiple Light Scattering

The potential of using multiple light scattering methods to monitor the state and concentration of photosensitizer and hemoglobin in whole blood is demonstrated. Samples of whole blood with various contents of chlorine e 6 photosensitizer were studied in the spectral region of 620–850 nm. On the basis of experimentally measured coefficients of diffuse reflection and transmission, absorption spectra of blood with and without the photosensitizer and the concentrations of oxy-, deoxy-, and methemoglobin in the samples are calculated. On the basis of this information, the absorption spectrum of chlorine e 6 in whole blood is reconstructed and its state and concentration are estimated for the first time. It is found that chlorine e 6 in whole blood occurs mainly in the monomer form.