Анализ выхода ДНК при ее автоматизированной экстракции из лейкоцитов крови

The continuing development of mass DNA extraction methods entails the need to set standardisation and quality verification reference points. A study has been conducted and standards set for one of the many methods of DNA extraction - the automated мagnetic beads-based extraction using QIAsymphony SP station. It was shown that the concentration of DNA in an individual sample is mainly determined by the leukocyte content in the initial blood sample (correlation of about 0.9). DNA concentration also depends on the measurement method. It allowed us to build linear regression models and derive formulae that accurately predict the concentration of DNA in the sample for the use of two widely used methods - spectrophotometric (Nanodrop) and fluorescence (Qubit). It was found that the latest Nanodrop OneC model, thanks to a built-in algorithm for identifying impurities and adjusting the concentration, provides an even more accurate concentration estimate than Qubit 4.0. For a quick but rough forecast of DNA concentration, instead of regression models, standards calculated by us for reference values of the white blood cell count can be used.