Development and validation of UV-spectrophotometric method for estimation of Baicalein in Oroxylum indicum leaf extract

A simple, precise and cost effective UV- visible spectrophotometric method for the estimation of Baicalein in Oroxylum indicum leaf extracts was developed and validated according to the ICH Q2 (R1) guideline. Baicalein (5, 6, 7- trihdroxyflavone) is a flavone isolated from the leaves of Oroxylum indicum plant. Spiked Baicalein solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of Baicalein were prepared. Calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters viz. linearity, accuracy, precision, robustness, ruggedness, limit of detection and limit of quantitation were calculated. The maximum wavelength of Baicalein was found to be 274 nm. The correlation coefficient over the concentration range of 0.5-8 μg/ml was found to be 0.999. Developed UV method was found to be precise for the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.498 & 1.809 to 0.809 to 1.87 respectively. The total percent recovery of Baicalein was found to be 98.96 to 99.27%. A simple, precise and cost effective UV- visible spectrophotometric method for the estimation of Baicalein in standardized extract of leaves of Oroxylum indicum was developed. The said method was developed using solvent containing economical percentage of organic phase in aqueous media. The present validated UV- visible method can be efficiently used for the estimation of Baicalein in extracts of leaves Oroxylum indicum.