Synthesis of non-natural DNA using DNA polymerase.

Nonnatural nucleotide modified by glucose or galactose was synthesized to increase functional diversity of DNA library. These compounds were incorporated in a DNA double strand using Klenow Fragment as well as dTTP. These functional group could be ordered sequentially on a DNA double strand at intervals of few angstroms according to the designed template sequence within a few hours. This method must be useful to constructing nonnatural DNA library or designed supramolecular structures. INTRODUCTION Various combinatorial libraries were constructed to create novel catalysis or receptor since 1990s. Peptide, polysome, Phage, RNA libraries indicated great success to the purpose. A number of DNA enzymes were also discovered through in vitro selection, but