Preparation method and application of affinity chromatography porous medium with glutathione ligand

The present invention relates to a method and a porous medium by affinity chromatography on a glutathione ligand band. Glutathione ligand to a radical transfer agent, with clever synthesis of glutathione affinity chromatography ligands porous media, the production of the base, processing shaped supporting medium and the carrier ligand-step reaction was complete. Using inexpensive base material, greatly reducing the cost. The affinity chromatography media material is porous open-cell structure, a pore size of about 100 ~ 200μm, the amount of bonded ligands glutathione key up 75.3mg / g, high bonding efficiency and high purity, the results show that affinity chromatography porous medium has good separation capability protein, glutathione -s- transferase (GST) binding efficiency of 1.5mg / g. Fusion protein using affinity chromatography column packed with a porous medium glutathione ligand prepared, can be used for glutathione transferases -s- -s- or glutathione transferase enzyme as the label separation and purification.