Electrical stimulation and millisecond-class ultralow temperature rapid freezing method and device thereof

The present invention relates to the technical field of an electron microscope and ultralow temperature rapid freezing. Disclosed is an electrical stimulation and millisecond-class ultralow temperature rapid freezing device, comprising: a sampling stage (3), a vertical sliding plate (5), an ultralow temperature freezing room (7), an infrared sensor A (9), an infrared sensor B (10), a timer (11), a programmable stimulator (14) and a computer (15). The sampling stage (3) is arranged on the vertical sliding plate (5). The infrared sensor A (9) and the infrared sensor B (10) are arranged respectively at an upper end and a lower end of the vertical sliding plate (5). The infrared sensor A (9) is arranged at an upper end of the sampling stage (3). The infrared sensor B (10) is at an equal altitude to an inlet (6) of a freezing room of the ultralow temperature freezing room (7). The two infrared sensors (9, 10) are connected with the timer (11). The device can enable a biological tissue to be frozen and fixed rapidly by the ultralow temperature upon being stimulated, so that morphologic change of an ultrastructure of the biological tissue in various specific situations when a physiological function changes can be studied, and studies of the physiological function and the morphology can be performed simultaneously at a millisecond level, which is significant for the development of subjects such as the electron microscopy, the cell ultrastructure morphology, the physiology, and the pathology.