[Cytogenetic analysis and bcr/abl mRNA detection in chronic myeloid leukemia].
1999
Objective To define the relationship between Ph chromosome
and BCR/ABL mRNA expression in chronic myeloid leukemia (CML) patients, and compare
cytogenetic analysis and PCR method. Methods Marrow or peripheral blood samples from 33
CML patients were analyzed by R banding technique and PCR method. Eleven patients who
received allogeneic BMT or α IFN+low dose HU were followed up. Results In 33 patients, 29
were Ph(+), 30 expressed BCR/ABL mRNA. In 6 patients who received BMT, 4 were Ph(-), 2 were
Ph(+). However, 2 patients were (-), 4 were (+) by PCR method. In 5 patients who received α
IFN, the percentage of Ph(+) cells reduced after 6 and 12 months treatment than before
treatment in 2 cases respectively. In 41 samples, 34 cases were consistent by 2 methods.
Conclusion Each method, Cytogenetic analysis or PCR has its advantage and defect. The better
method are integration of 2 methods in diagnosis and treatment monitoring of CML patients, so
that provide real and beneficial information for clinic.
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