ЗМІНИ ВМІСТУ С-РЕАКТИВНОГО ПРОТЕЇНУ ТА ФАКТОРУ НЕКРОЗУ ПУХЛИН-α У СИРОВАТЦІ КРОВІ ЩУРІВ РІЗНОГО ВІКУ ЗА УМОВИ ПАСИВНОГО ТЮТЮНОКУРІННЯ НА ТЛІ ТРИВАЛОГО ВВЕДЕННЯ НАТРІЙ ГЛУТАМАТУ

The World Health Organization considers tobacco smoke as a global health and social problem. The active use of food supplements is ambiguous as regards human health and safety. So, the study of molecular mechanisms of toxicity of isolated and combined action of tobacco smoking and monosodium glutamate is one of the topical issues of modern science. The study aimed to determine changes in the C-reactive protein and tumor necrosis factor-α of blood serum in rats under secondhand tobacco smoking combined with a long-term monosodium glutamate injection in the age aspect. Experiments were carried out on 64 outbred white male rats divided into the following groups: I – control group; II – rats with modeled secondhand tobacco smoking; III - rats, which were injected with monosodium glutamate; IV - rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection. Blood serum TNF-α and CRP content were performed by enzyme-linked immunosorbent assay (ELISA) Kit developed by RayBiotech (USA) following the manufacturer’s protocol on a Multiscan FC analyzer (Finland). The findings suggest that under the combined action of secondhand tobacco smoke and monosodium glutamate, the content of TNF-α increased by 2.9 times (p<0.001)) vs control group and by 32.3% (p<0.002) exceeded the data under the isolated action of secondhand tobacco smoke. Regarding the CRP, this indicator increased by 45.5% (p <0.001) vs control group. Secondhand tobacco smoke combined with monosodium glutamate use did not cause significant changes vs the isolated effect of tobacco smoke. As regards age, the systemic inflammatory response was more pronounced in immature rats.