Construction of aptasensor for sensitive detection of 8-OH-dG based on diffusion mediated electrochemiluminescence quenching effect

A DNA immobilized-free ECL aptasensor was developed for the detection of 8-hydroxy-2´-deoxygunosine based on the diffusion mediated ECL quenching effect. This ECL aptasensor exhibited a high sensitivity and low detection limit by combining homogeneous DNA reaction with dual signal amplifications: target-induced muti-DNA release and Exo Ⅰ-assisted target recycling.