Ultrafast laser scanning cellular microscopy by spatiotemporally encoded virtual sources

We report a new type of all-optical ultrafast laser-scanning microscopy( (at a line-scan rate of 20 MHz) based on a phenomenon called free-space angular-chirp-enhanced delay (FACED). It results in the generation of a reconfigurable array of spatiotemporally encoded virtual pulsed sources, which acts as a scanning laser beam. We demonstrate its application in high-throughput multivariate image-based single-cell analysis (10,000 cells/sec).