Fungistatic Activity of Crude Protein Extracted from Bacillus subtilis HJX1

Bacillus subtilis HJX1,a biocontrol strain of Fusarium oxysporum f. sp. cubense,could inhibit growth of several plant pathogenic fungi in vitro. To understand its mechanism of fungistasis,we extracted crude protein from fermentation broth of the strain HJX1 through ammonium sulfate precipitation,and preliminarily studied fungistatic activities at different conditions. The results showed that the crude protein was insensitive to protease K,trypsin or ultraviolet radiation. The fungistatic activity was unchanged when treated in water batch at 40 ℃,60 ℃,80 ℃ or 100 ℃ for 30 min,and the fungistatic activity maintained 60% when treated at 121 ℃ for 30 min.