Adhesion and locomotion of L5222 cells on endothelium, collagen and glass.

SUMMARY Examination was made of the adhesive interaction of L5222 leukaemia cells with endothelial cells, collagen and glass and of cell locomotion on endothelium and collagen. Leukaemia cells interacted with the substrate under stationary conditions. The fraction of cells adherent to the substrate was defined next, using the given shearing force caused by the medium flowing through the measuring channel. The relative number of adherent cells, A (related to the number of cells after sedimentation), remaining on the given surface despite the detaching action of shearing force, F, was determined. The range of F values applied was o-i to 30 ( x io~ 13 ) N. It was found that the relation A(F) is a decreasing function for all the substrates examined and takes on values in the relation 1:2:6 for cells adherent to collagen, glass and endothelium, respectively. The critical value of F, at which values of A are maintained at a constant level close to zero, was 0-5, 1 and 3 (x io" 11 ) N, respectively, for cells adherent to these substrates. The function A(JF) for L5222 cells adherent to endothelial cells and to glass can be described well by the formula: A = a/sIF (where a = constant > o). Studies of L5222 cell locomotion on an endothelial cell layer and on collagen revealed that the pattern of locomotion, variations in locomotion velocity and the mean values of cell displacement (S'8 and 5-0 fim, respectively) are similar for both substrates.