Intratumoral vaccination with activated allogeneic dendritic cells in patients with newly diganosed metastatic renal cell carcinoma (mRCC).

3085 Background: Accumulating data indicate that the efficient induction of antigen-specific CTLs characterizing viral infections is caused by cross-priming where infected DCs produce an unique set of inflammatory factors that recruit and activate non-infected ”bystander” DCs. Accordingly, we have developed a cellular adjuvant consisting of activated DCs producing high levels of DC-recruiting and DC-maturating factors in a sustained fashion. This concept doesn´t require MHC-compatibility between injected cells and the patient and therefore introduces the possibility of using pre-produced and freeze-stored DCs from healthy blood donors as an ”off-the-shelf” anti-tumor vaccine when injected intratumorally. Methods: 12 patients with newly diagnosed mRCC (5 with poor prognosis according to Heng criteria) where included in a clinical phase I/II study. Vaccine cells (50 doses) were produced from a leukapheresis-product from one healthy blood donor and subsequently deep-frozen. 5-20 x 10(6) vaccine cells were in...