Organogenetic differentiation in callus tissues of Acacia senegal (L.) willd.

Callus from hypocotyl and cotyledon explants of Acacia senegal was raised on MS-medium supplemented with 2,4-D (2.5 mg/l) in combination with BAP (0.5 mg/l). When transferred, differentiated into shoot buds on MS + BAP (2.5 mg/l) and Kn (2.5 mg/l) medium. Other additives viz, adenine sulphate (25.0 mg/l) ascorbic acid (10.0 mg/l) and glutamine (146.0 mg/l) were also added to the medium. The shoot buds elongated and developed on MS-medium with lower concentration of BAP and Kinetin (0.5 mg/l each). Rooting of regenerated shoots was achieved on 1/4 strength MS-medium fortified with IAA (3.0 mg/l).