Generating modified polymerases for improved accuracy in sequencing a single molecule

A composition comprising a recombinant DNA polymerase, recombinant DNA polymerase comprising an amino acid sequence that is at least 80% identical to SEQ ID NO: 1, recombinant DNA polymerase comprising a glutamic acid residue, a residue of aspartic acid , a histidine residue, a lysine residue, an arginine residue or a tyrosine residue in the A484 position, in which the identification of the positions is with respect to SEQ ID NO: 1 and DNA polymerase recombinant showing polymerase activity.