Sodyum hipoklorit, klorheksidin ve propolis içerikli solüsyonların potasyum titanyum fosfat lazer ile birlikte kullanımlarının candida albicans üzerine etkinliklerinin incelenmesi

Aim: Antimicrobial effects of sodium hypochlorite, chlorhexidine gluconate (CHX) and different concentrations of propolis solutions (PS) were evaluated on Candida albicans in root canals. In the next step, after application of irrigation solutions antimicrobial effect of Potassium Titanium Phosphate (KTP) laser against C. albicans was tested. Material and Methods: Freshly extracted forty-two single rooted human teeth were divided into 6 equal groups; And selected irrigants and application were for Group 1 NaOCl, Group 2 CHX, Group 3 5%PS, Group 4 10%PS, Group 5 20%PS, Group 6 Saline solution (control group). Roots with predetermined lengths were placed in glass bottles. Then, the roots were shaped using ProTaper files by crown down technique. Then the teeth sterilized by ethylene dioxide and contaminated with C. albicans. After then, 2 ml of irrigation solution was used for each teeth. 24h growth were determined by evaluation of turbidity in test tubes. As a final step, KTP laser was applied to root canals and microbial growth were determined. The results were evaluated using Kruskal Wallis, Mann Whitney-U, Chi square and Friedman tests. Results: All tested irrigation solutions (except for control group) were found statistically effective against C. albicans with regard to antimicrobial efficacy (p<0.05). After having done irrigation in the root canals, application of KTP laser with propolis %20, propolis %10 and SF was found statistically effective with regard to antimicrobial efficacy (p<0.05). Conclusion: In root canals, propolis had antimicrobial effect, however results were unsatisfactory. Furthermore, addition of KTP laser energy gave stronger antimicrobial effect. OZET Amac: Kok kanalina uygulanan, sodyum hipoklorit (NaOCl), klorheksidin glukonat (KHG) ve farkli konsantrasyonlarda hazirlanmis propolis solusyonlarinin (PS) Candida albicans uzerine antimikrobiyal etkinliklerinin karsilastirilmasidir. Ayrica Potasyum Titanyum Fosfat lazerin (KTP) irrigasyon solusyonlarindan sonra uygulanmasinin C. albicans uzerine antimikrobiyal etkinligini degerlendirmektir. Gerec ve Yontem: 42 adet yeni cekilmis tek koklu insan disi, her biri 7 disten olusan alt gruplara ayrildi. Grup 1 NaOCl, Grup 2 KHG, Grup 3 %5’lik PS, Grup 4 %10’luk PS Grup 5 %20’lik PS, Grup 6 Serum fizyolojik (kontrol grubu). Calisma boyutlari hesaplanan disler lastik kapakli cam siselere yerlestirildi. ProTaper kok kanal aletleri ile crowndown teknigi kullanilarak prepare edildi. Cam siselerdeki steril disler aminar air flow’da C. albicans’la kontamine edildi. Her dis icin 2 ml irrigasyon solusyonu kullanildi. 24 saat sonra bulanikliklarina bakilarak sonuclar degerlendirildi. Son asama olarak kok kanallarina KTP lazer uygulandi ve mikrobiyal ureme olup olmadigi belirlendi. Veriler degerlendirilirken Kruskal Wallis testi, Mann Whitney-U testi, Chi square, Friedman testi ve Wilcoxon testi uygulanmistir. Bulgular: Tum test edilen irrigasyon solusyonlarinin (kontrol grubu haric) C. albicans’a karsi antimikotik etkinlik yonunden istatistiksel olarak onemli bulundu (p<0,05). Propolis %20, Propolis %10 ve SF (kontrol gurubu) ile kok kanallarinda irrigasyon yapildiktan sonra KTP lazer uygulamasi antimikotik etkinlik yonunden istatistiksel olarak onemli bulundu (p<0,05). Sonuc : Kok kanallarinda, propolisin, antimikrobiyal etkisi olmasina ragmen istenilen yeterlilikte bulunamadi. Ayrica irrigasyon solusyonlarindan sonra, KTP lazer uygulanmasinin daha etkili antimikrobiyal etki gosterebilecegi belirlendi. Anahtar kelimeler: Propolis, irrigasyon, lazer, mikroorganizma