The Korean Soclety of Plant Pathology : K ; Virology and Virus Diseases : K-18 ; Development of a standard curve for detection of the Polymyxa graminis population using qRT-PCR

Polymyxa graminis is an obligate root-infecting plasmodiophore initially described from wheat root cells. It is the responsible vector for transmit-ting the Barley yellow mosaic virus (BaYMV). P. graminis damages to not only barley plants but also to cereals and grasses. It can survive in soil also as restingspores in the form of thick-walled clusters. Under favourable conditions they develop biflagellate zoospores to infect barley root cells and survive within. The precise and expedient methods to assess the den-sity of P. graminis in soil and host plant tissue are necessary to diagnose the infection and draw the correlation between pathogen density and pathogenicity. Here, we are trying to develop the simple way to estimate of P. graminis resting spores population in barley root tissue. A standard curve would be generated as the function of P. graminis resting spores and Ct values deduced from realtime RT-PCR. For development of stan-dard curve conditions (spore count/ml or spore count/gm) are going to optimized. For this standardization all the P. graminis samples were taken from BaYMV infected roots rather than soil. Current study will help us to get more information on Polymyxa population present, vector etiology as well as disease management in a roundabout way.