GW24-e2346 PM2.5 affect RAS Gene Expression in Human Vascular Endothelial Cell

Objectives To observe the effect on RAS gene expression of different components of PM 2.5 on human umbilical vein endothelial cells (HUVEC) and to explore the potential mechanism. Methods PM 2.5 in the atmosphere of Changsha city was collected through 2010-2011. The vascular endothelial cells HUVEC-12 were treated by soluble and insoluble components of PM 2.5 (exposure doses: 100 μg/ml, 200 μg/ml and 400 μg/ml, respectively for each two kinds of components) . For the intervention group, HUVEC was exposed to the 400 μg/ml soluble and insoluble extracts of PM 2.5 , respectively, followed by an intervention of Perindopril in concentration of 10 μmol/l. Cell activity was detected by MTT colorimetric assay. Furthermore, such RAS genes as ACE, ACE2, AT1R and Mas were observed using quantitative RT-PCR. Results The results showed that the soluble and insoluble components of PM 2.5 caused the decrease of cell survival rate in a dose-dependent manner (P Conclusions Diverse components of PM 2.5 caused different RAS gene expression in vascular endothelial cells. Compared with the soluble components, insoluble components showed more severe toxicity.