Experimental evaluation of angularly-variable fiber geometry for targeting depth-resolved reflectance from layered epithelial tissue phantoms
2007
The aim of the present study focuses on experimentally demonstrating the efficacy of
using angularly-variable fiber geometry to achieve the desired tissue-layer selection and
probing depths with the further objective of enhancing the sensitivity and specificity of
spectral diagnosis in stratified architectures that resemble human cervical epithelia. The
morphological and biochemical features of epithelial tissue vary in accordance with
tissue depths; consequently, the accuracy of spectroscopic diagnosis of epithelial
dysplasia may be enhanced by probing the optical properties of this tissue. When
correlated to cellular dysplasia, layer-specific changes in tissue optical properties may be
deciphered by reflectance spectroscopy coupled with angularly-variable fiber geometry.
This study addresses the utility of using such angularly-variable fiber geometry for
resolving spatially-specific spectral signatures of tissue pathology. This is accomplished
by interpreting and analyzing the reflectance spectra of increasingly dysplastic epithelial
tissue in two-layer epithelial phantoms. Spectral sensitivity to tissue abnormalities in the
epithelial layer is significantly improved as the obliquity of the collection fibers increases
from 0 to 40 degrees. Conversely, conventionally orthogonal fibers are found to be more
sensitive to changes in stromal tissue properties.
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