The effect of the bacterial pathogens Staphylococcus aureus and Streptococcus uberis on morphological features of apoptosis of heifers mammary gland neutrophils

The aim of the student thesis was to review the effect of the significant bacterial pathogens, Staphylococcus aureus and Steptococcus uberis on programmed cell death - apoptosis in vitro, and to determine the dynamic of morphological changes during aging neutrophil granulocytes of heifers mammary gland in vitro. Using light and electron microscopy we have noted characteristic alterations of apoptotic neutrophils. These are running in three consequential phases. We found out, that the interaction of bacterial pathogens with neutrophils during the incubation have lead in expressive quantitative and qualitative changes in apoptotic cells proportion. Concretely, the influence of both pathogens on mammary gland neutrophils caused the defer of apoptosis expression. Here, S. aureus caused lower number of apoptotic neutrophils in comprasion with S. uberis. The outcomes testified, that S. aureus and S. uberis interaction with heifers mammary gland neutrophils in vitro causes alterations relating to apoptosis of these cells. Looking at the results of the study, we can conclude, that the pathogens S. aureus and S. uberis are not only significant heifers mammary gland - affection causers, but they significantly influence the cells of defensive system in their functions too. They significantly decrease the appereance of morphological apoptosis manifestations on neotrophils of tissue pool of the heifers mammary gland. The numbers of apoptotic cells in neutrophil population confirm, that during the interaction with mentioned pathogens the defer of morphological apoptosis manifestations happens. Then, higher number of apoptotic neutrophils in stages of apoptotic corpuscles implies increasing dynamic of this process. Beside that, the dynamic of apoptotic process is influenced by the specifity of certain bacterious actor too.