Special properties of polycentric anaerobic fungus Anaeromyces mucronatus

Fliegerova K., S. PaIoutova, J. Mrazek, J. Kopean˘: Special Properties of Polycentric Anaerobic Fungus Anaeromyces mucronatus. Acta Vet. Brno 2002, 71: 441-444. The aim of this study was differentiation of polycentric rumen fungus of genera Anaeromyces using modern methods of molecular biology. Six polycentric anaerobic fungi isolated from cow, lama and bison were identified as Anaeromyces strains and analyzed for variability in internal transcribed spacers and fibrolytic activities. RFLP of both ITS1 and ITS1-4 fragments digested by restriction enzyme DraI separated studied strains into two groups with different cleavage profiles. Representatives of each group exhibited different hydrolytic enzyme activities. The largest differences were recorded in production of xylanase, β-endoglucanase and β-glucosidase. The possibility of using RFLP analysis of ITS regions to distinguish fungal isolates has been proved, however the results do not illuminate what kind of distinction is reflected in genetic variability of ITS regions.