Construcción de curvas dosis-efecto por irradiación de células mononucleares de sangre periférica analizadas con el ensayo cometa alcalino

The suitability of the alkaline comet assay to detect levels of DNA damage in human monocytes and lymphocytes under radiation was tested. For this purpose, four dose-effect curves by irradiation of cells isolated from blood samples of 4 young, health and non-somker donors were constructed. A gamma ray source of 60Co was used and doses of 0, 2.5, 5 and 7 Gy were delivered to blood cells. The results show good correlation (R2 = 0.973, SD = 0.009) between the radiation dose and DNA damage as measured by the parameter Tail Intensity (percentage of DNA in the tail of the "comet"). Furthermore, we observe the variability in predicting DNA damage by Gy (7.59, SD = 1.5). Potential use of this technique in the biological dosimetry of ionizing radiations is being evaluated.