Insect Cells and Larvae, Gene Expression Systems

Introduction Insect Cell Lines Viruses Baculovirus Gene Expression Construction of Recombinant Baculoviruses Posttranslational Processing Glycosylation Other Posttranslational Processes Applications Large-Scale Processing: Cell Culture or Larval Production Cell Culture Processes Bioreactors: Stirred-Tank versus Airlift Process Monitoring Techniques Oxygen Supply Cell Damage During Sparging Media Formulation Cell Culture By-products Batch Production Techniques Virus Stocks, Multiplicity of Infection, and Time of Infection Fed-Batch, Semibatch, and Sequential-Batch Production Continuous Production Systems Passage Effects and Mutant Virus Formation Larval Production Systems Conclusion Acknowledgments Bibliography Keywords: baculovirus; bioreactor; cell culture; expression system; glycosylation; insect; larva; recombinant DNA