Higher mean and fluctuating temperatures jointly determine the impact of the pesticide chlorpyrifos on the growth rate and leaf consumption of a freshwater isopod

Abstract There is growing evidence that both increases in mean temperature and the widespread daily temperature fluctuations (DTF) may increase pesticide toxicity. Nevertheless, the likely more stressful, realistic combination of the two warming-related stressors has rarely been considered in ecotoxicology. Moreover, we have little knowledge on whether these stressor combinations could impair ecosystem functioning. We examined the effect of the pesticide chlorpyrifos under an increased mean temperature (+4 °C, from 18 °C to 22 °C) and in the presence of DTF (constant and 8 °C) on two life-history traits (mortality and growth rate) and one ecologically important behavioural trait (feeding rate) in the freshwater isopod Asellus aquaticus. The chlorpyrifos concentration used, 0.2 μg/L, did not cause mortality in any thermal condition, nor did it cause sublethal effects at the mean temperature of 18 °C. A key finding was that growth rate was strongly reduced by the pesticide only under the combination of both a higher mean temperature and DTF, highlighting the importance of testing toxicity under this realistic thermal scenario. The leaf consumption of chlorpyrifos-exposed isopods increased at the higher mean temperature when this was kept constant, however, it lowered again towards control levels when DTF was induced, thereby contributing to the growth reduction at this most stressful condition. These alterations of growth and leaf degradation rates may impact nutrient recycling, a key ecosystem function. Our results highlight the importance of integrating both increases in mean temperature and in DTF to improve current and future ecological risk assessment of pesticides.