Enhanced and sustainable pretreatment for bioconversion and extraction of resveratrol from peanut skin using ultrasound-assisted surfactant aqueous system with microbial consortia immobilized on cellulose.
2020
In this study, the ultrasound-assisted surfactant aqueous system coupled with microbial consortia immobilized by cellulose has been created as an enhanced and sustainable method for the bioconversion and extraction of resveratrol from peanut skin. Based on central composite design, and several single-factor experiments, we derived the optimal bioconversion and extraction system. Microbial consortia consist of Yeast CICC 1912, Aspergillus oryzae 3.951 and Aspergillus niger 3.3148 were chosen to be immobilized using cellulose. Other treatment conditions include concentration of surfactant as 3% (w/v), temperature as 30 °C, time as 36 h, ultrasonic power as 250 W and liquid to solid ratio as 25:1 mL/g. Under these conditions, we achieved a promising yield of resveratrol 96.58 μg/g, which is 4.02 folds compared to the untreated sample. This sustainable and green method not only enhanced the production of resveratrol but also improved the safety and reliability of the bioconversion and extraction process. Our novel method has shown great potential to realize large-scale bioconversion and extraction of bioactive compounds from plant waste.
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