LC-MS/MS를 이용한 혈장중 vortioxetine 및 carboxylic acid 대사체 동시분석법 개발과 실제 복용자 혈장 중 함량 분석

One purpose of this study was to develop and validate a quantitative LC-MS/MS method for the analysis of vortioxetine and its carboxylic acid metabolite, and the other purpose is to obtain a therapeutic range of vortioxetine by applying it to the plasma of the actual medicine takers. The targetcompounds were measured with liquid chromatography-tandem mass spectrometry (LC-MS/MS) after deproteination. The LC system was coupled to a 4500 QTrap mass spectrometer using electrospray ionization in positive mode and multiple reaction monitoring (MRM) mode. The column was Kinetex XBC18 column. The method showed acceptable precision and accuracy, which were less than 12%, as well as satisfactory matrix effects and recoveries. The concentrations of vortioxetine and carboxylic acid of vortioxetine in plasma of the actual medicine takers were 3.23 ng/mL ~ 63.8 ng/mL and 3.63 ng/mL ~ 46.9 ng/mL, respectively. In conclusion, this LC-MS/MS method can be a sensitive and specific protocol for determining vortioxetine and its carboxylic acid metabolite in human plasma. Also vortioxetine concentration results of actual medicine takers can be considered as a good reference when evaluating vortioxetine toxicity.